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Even though sequences During this database are fully covered from the Refseq consultant genomes databases, it doesn't comprise the alternate loci and so avoids sequence redundancy introduced by together with alternate loci. This database is recommended if You aren't considering versions represented by alternate loci.
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Common sequences of Brief DNA oligonucleotides are frequently used as primers for sequencing cloned genes (Sanger sequencing). Choose between our usually employed sequencing primers to initiate your sequence verification projects.
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This is useful for restricting the amplification only to mRNA. You may as well exclude these kinds of primers if you'd like to amplify mRNA along with the corresponding genomic DNA. Exon junction match
In contrast to random primers, oligo (dT) get more info primers will not be suited to degraded samples but are superb for setting up cDNA libraries and advisable for use with reverse transcriptases (RTs) as Superscript IV RT.
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e., toward end of your primer) of the exon-exon junction. Annealing to both equally exons is critical as this assures annealing into the exon-exon junction area but not either exon by yourself. Take note that this option is powerful only if you select "Primer ought to span an exon-exon junction" for "Exon junction span" option. Intron inclusion
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